"We're expecting bookings for our online Virtual Penguin Encounters to fill up fast as this will give our followers a great insight into the various characteristics of their favourite penguins and may confirm who they'll vote for."
The 14 'Little Penguins' all originally arrived at the aquarium having been abandoned as chicks, or been sick or injured.
While some penguins can return to the wild after recovering, these ones became permanent residents as they were not strong enough to return to their natural habitat.
Beyond the two previous champions, one of the favourites to take the title is 2019 finalist Mo, who was awarded the Penguin of the Month award for September by the penguins' keepers for consistently being the first to go swimming in the morning.
The initial round of voting opened on Thursday, and will run on the National Aquarium website until next Friday, October 9, when three finalists will be named.
Another round of voting between that trio will see the ultimate winner crowned on October 15.