The deputy director of the Waikato University laboratory, Dr Fiona Petchey, said lab staff were unaware of their involvement in the New South Wales cold case, as they were given limited details on samples.
But it was not unusual for the laboratory to test human bone as part of the university's work for commercial clients, including police.
Radiocarbon dating uses levels of the radioactive carbon isotope C14 in organic material to determine its age.
"Because it decays over time and does so at a set rate, it ... enables us to go directly into the sample and measure the amount of C14, and then calculate how long since that sample stopped interacting with the atmosphere," Dr Petchey said.
The age was then calibrated according to fluctuations of carbon in the atmosphere over time, she said.
Samples since 1950 were calibrated on the "bomb curve", based on changes of carbon in the atmosphere caused by nuclear bomb testing.
"In a very short period of time there was an enormous spike in C14 in the atmosphere, that transferred into the oceans and into the soil."
Atmospheric carbon levels had been constantly measured since that time, giving accurate data for carbon dating scientists to work from.
New South Wales police said samples of Mrs Bartlett's remains were also sent to Texas for DNA testing.
Scientific evidence had contributed to the conclusion her death was suspicious, but police would not reveal further details.