If found guilty the woman could also face up to five years behind bars thanks to a bill passed in December, which introduced a specific offence for defacing or damaging cultural heritage.
According to Italian media, 20 people have been charged for vandalising the Colosseum in the last three years and a further ten have been accused of trespassing on the landmark.
The Colosseum, where Roman gladiators once took part in gruesome battles, underwent a €25 million ($36.9 million) restoration last year, which was largely funded by the Italian fashion brand Tod.
The cash-strapped Eternal City has increasingly been turning to private investors - often luxury fashion brands - to help fund the restoration of popular landmarks.
The Italian fashion house, Bulgari, recently put up €1.5 million ($2.2 million) to help restore the famous Spanish Steps, while Rome-based Fendi helped sponsor renovation work at the Trevi Fountain.
This article originally appeared on the Daily Telegraph.