The reasons for removing the letters seem to be ostensibly political. City council leaders deemed the sign to be "too individualistic" and in opposition to "progressive values."
Femke Roosma, a councillor for the city's biggest party - "Green Left" – said in a statement : "The message of "I Amsterdam" is that we are all individuals in the city. We want to show something different: diversity, tolerance, solidarity."
However the removal of the sculpture might be a sign of the increasing pressures of tourism felt by Amsterdam natives.
During the fourteen years the sign was in place annual tourism figures have bloomed from 4.2 million in 2004 to a projection of almost 19 million this year.
For some of the 741,000 natives it can feel as if their city has been hijacked by visitors.
But not all the Dutch residents are pleased to see the sign go.
Talking to the MailOnline Frits Huffnagel, who came up with the concept for the sign, explained he was shocked by the signs disappearance.
"The Greens don't understand a thing about the campaign," he said.
"I am part of Amsterdam. As a citizen, because I work there, or as a visitor. That together makes Amsterdam. That everyone wants to belong to it. It is connecting people, you gather people under that slogan."
Perhaps those who are most shocked to find the sign missing are tourists. The city council has had to hire "hosts" to explain the removal of the sign to bemused visitors looking for a holiday snap.
In the empty place previously occupied by the sign protesters have placed their own sign, expressing a mortified, orange "Huh?"
