The Geraldine Fairlie Highway remains partially blocked near Mt Michael and Pusey Rd, where the bus ran off the road, toppling over on its side.
The police spokeswoman said eyewitnesses reported the bus lying half in and out of the ditch on its side.
The highway between Geraldine to Fairlie was now down to one lane and motorists were warned to take extra care near the Mt Michael Valley Rd intersection.