The site rises 20m above the surrounding area, with a clear view across farmland to the Anzac approach route. It was a key position overlooking the strategic township of Beersheba, which was guarded by Turkish forces. Anzac forces captured the town just before sunset - the first step in an offensive that led to the capture of Jerusalem, six weeks later.
Australian Prime Minister Malcolm Turnball and Opposition leader Bill Shorten will also attend the centennial.
Dame Patsy and Sir David will leave New Zealand tonight, and the commemoration will take place on Tuesday. It will include a joint Anzac service at Be'er Sheva War Cemetery, the opening of an Anzac Museum, and the New Zealand commemoration.
Dame Patsy will also visit the United Arab Emirates and the occupied Palestinian Territories during her trip, as well as open the New Zealand art exhibition Sand in the Apricot Jam at the Museum of Rishon LeZion.