"Aspergillosis is a very susceptible disease and causes severe damage to the lungs and air sacs in birds. It is also incredibly difficult to treat with a very low success rate,'' she said.
The bark which the birds were housed on had been used regularly at the zoo to stimulate a natural environment.
There had been no prior problems, Ms Macpherson said.
"Obviously zoo and DOC staff are devastated by the loss of the birds.''
It would also be a "big blow'' for charity Kiwis for Kiwi, which has been working with DOC to increase Rowi numbers through the BNZ Operation Nest Egg programme.
"These birds were removed from the wild as eggs in order to protect them while they grew big enough to defend themselves.''
After living on predator-free Motuara Island in the Marlborough Sounds, they were transferred to the Wellington Zoo and Massey University in September for specialist treatment, Ms Macpherson said.
DOC was also waiting for autopsy results for another Kiwi death which occurred at Massey University. It was unrelated to the eight deaths at Wellington Zoo, she said.