Ms Palmer said pet dogs could also kill kiwi.
"Even dogs that are very placid in nature have been known to kill kiwi."
She said kiwi were also vulnerable to vehicles as they crossed roads to neighbouring habitat.
"Wairere, the 2-year-old kiwi found on the side of the road just below Kohi Point last week, is well known by Kiwi Project staff and volunteers. She was blessed at a ceremony held at Ohope Beach School on the 20th January 2011 and later released into Kohi Point," Ms Palmer said.
"I knew it was only a matter of time before one of our kiwi was hit by a car. I had hoped I would be wrong. It would be devastating if all the hard work undertaken during the last 11 years by Whakatane Kiwi Project partners and volunteers was undone because people didn't take the Kiwi Crossing or Kiwi Zone signs seriously."
Ms Palmer said she hoped the latest deaths would help raise awareness about the endangered national bird.
"The key is that dog owners need to be responsible and keep their pets on a lead. People need to understand that kiwi can be half a metre off the track or road sheltering under a bush," she said.
"Kiwi have a very strong scent so dogs can find them by day or night and they are defenceless against dogs."