"Tobey and I are basically the same age - and I was told I was too old to play her. I'm like, 'Oh OK, that's what I've signed up for'."
Banks instead had to settle for a much smaller role in the film, playing Betty Brant, a secretary at the Daily Bugle - a role she reprised in two more Spider-Man films.
In the ensuing years, Banks has forged a career as a Hollywood power player both in front of and behind the camera, with roles in 40-Year-Old Virgin, The Hunger Games franchise and The Lego Movie. She produced both Pitch Perfect films, directing the sequel.
Banks told Glamour she feels she's laid the foundations for a long Hollywood career.
"What I'm grateful for now is longevity. I was never a flavour of the month. I feel very comfortable that I will be working in this industry for a while," she said, explaining her heart lies in comedies.
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"I love character-based comedies. It's important to have a view on the world that's a little sassy. I don't think we should be taking anything too seriously."
Banks, a mother of two, this month jokingly dubbed herself a 'f**king a**hole' after her Hollywood duties forced her to miss her son's preschool graduation.