The tweet prompted hostile reaction on social media, including from Ella Ceron, whose bio describes her as digital entertainment editor at teenvogue.
"I would call you out on this @MTVAUSTRALIA but as I'm Latina, you might not understand what I'm typing here," she tweeted. "P.s. America and Eva were *born in* the US so they don't have foreign accents, people trying to explain away this joke!"
Other social media users also took MTV to task.
MTV Australia said it will review its editorial guidelines with staff to prevent a similar situation in future.
"The tweet in question was an attempt at sarcasm that, quite simply, missed the mark," it said in a statement.
"It was never intended to be taken seriously nor to offend anyone as there was obviously no need for subtitles in the segment.
"Out of context it is clear that the comment could be misunderstood, and we sincerely apologise for any offence taken."
MTV's editorial guidelines stipulate it must not post material considered judgemental, prejudicial or intentionally offensive to someone based upon their religion, race or culture.
-AAP