"The TPP is an imposition on our parliamentary sovereignty to make laws that protect the public interest," said Amanda Vickers, from opposition group TTP Free Wellington.
Organisers say they also hold concerns about the effect of the TPP on Maori.
On Saturday, Wellington iwi Ngati Toa and Te Atiawa will gather at Waitangi Park on the capital's waterfront and march to Government House, where they meet the main hikoi.
The marchers' petition asks the Governor-General not to sign into law any legislation about the TPP unless there has been a binding referendum.
"No one can force the Governor-General to sign legislation," Ms Vickers said. "If he doesn't sign it, it doesn't become law."