Kim Dotcom's assets will be within reach of the United States before the entrepreneur's extradition process is completed.
A Court of Appeal decision, released today, means millions of dollars, several luxury cars, jewellery and other property remain frozen.
The ruling is also a blow for Mona Dotcom, who is now separated from Dotcom and unsuccessfully applied for her property to be excluded from the order.
Ms Dotcom had argued that she had not been charged with any offence and had no knowledge that any property was the proceeds of criminal activity.
The US government's attempts to have Kim Dotcom extradited to face trial on copyright infringement, money laundering and racketeering charges relating to file-sharing website Megaupload have been delayed by numerous court battles.