Despite being initially welcoming and allowing the wild animal into their house, the neighbours became increasingly suspicious of Neo's behaviour.
He avoided eye contact with them and would hide in the bathroom and did not show interest in dog treats.
Eventually, they decided to take him to the Humane Society of Southern Arizona who told them that the creature was not a normal dog.
Neo not only looked like a wolf but also demonstrated the behaviour of the non-domesticated animal. Pictures show him pulling at the leash, not making eye contact with humans and behaving like a pack animal.
The organisation's former CEO Maureen O'Nell said she told them: "You know that isn't a dog, right?"
The couple told her that they had been "wondering".
She contacted Wolf Connection to ask whether they would take him in and after they agreed she spoke to his owner about whether he would be willing to give him up to the sanctuary.
When he agreed, Maureen said she was "proud of his decision," adding: "His boy had a wonderful life ahead of him at Wolf Connection."
The sanctuary put Neo in isolation when he arrived but they said he quickly escaped to find their alpha female Maya and on his first night joined the "nightly howl".
A video shows him energetically playing at the sanctuary with another wolf, Nova, when he arrived there nearly two years ago.