Rouhani, who fronted Tehran's deal with six major powers in 2015 to curb Iran's nuclear programme in return for the lifting of sanctions, said the US Administration lacked knowledge about the Middle East.
"Americans resorted to many different methods against Iran but failed in all ... We are waiting for the new US Administration to find stability and continuity in its policies," Rouhani said. "The problem is that the Americans do not know our region and those who advise US officials are misleading them."
Rouhani said Iran was the vital force behind the fight against Isis (Islamic State) in Iraq and Syria and repeated Iran's official stance that the US and Saudi Arabia are funding "terrorism" in the Middle East.
"Who fought against the terrorists? It was Iran, Syria, Hizbollah and Russia. But who funded the terrorists? Those who fund terrorists cannot claim they are fighting against them," he said.
Tehran and Riyadh are involved in proxy wars across the region, backing opposite sides in Syria, Yemen, Iraq and Lebanon.
Already fragile diplomatic and trade ties between Sunni Saudi Arabia and Shia-dominated Iran were severed last year, after Saudi Arabia executed a Shia cleric and as a result protesters ransacked the Saudi embassy in Iran.
He said Iran welcomed better relations with its regional neighbours and pledged to fulfil his campaign promises of opening Iran to the world and delivering freedoms to the Iranian people.
"The Iranian people voted for moderation as they know a prosperous economy and jobs can only happen through investment, and investment through freedom and interaction with the world," he said.
- Reuters