Netanyahu cautioned against allowing Iran to retain even a "residual" capacity to enrich uranium, a process that could be used to make fuel for nuclear power stations, which Tehran says is the only goal, or a nuclear weapon core.
"Israel will never acquiesce to nuclear arms in the hands of a rogue regime which repeatedly threatens to wipe us off the map. If Israel is forced to stand alone, Israel will stand alone. But, in standing alone, Israel will know that it is defending many, many others," said Netanyahu.
He recalled how Iranian-sponsored terrorists destroyed a Jewish community centre in Buenos Aires in 1994, killing 85 people, and Khobar Towers in Saudi Arabia, killing 19 American soldiers in 1996.
"Are we to believe that Rouhani, the national security adviser of Iran at the time, knew nothing about these attacks?" asked Netanyahu. "Of course he did."
Saying that Rouhani negotiated with the West while expanding Iran's nuclear facilities a decade ago, Netanyahu added: "He fooled the world once, now he thinks he can fool it again."
Khodadad Seifi, a deputy ambassador at Iran's UN mission, dismissed Netanyahu's remarks as "inflammatory" and "sabre rattling".
Western diplomats believe privately that Netanyahu's definition of an acceptable agreement with Iran is unrealistic. One described his conditions as an "unconditional surrender".