Andreescu played it down post-match, suggesting she did not hear what the German said.
"She said something, but I'm not sure what," Andreescu said.
"I just didn't say anything, whatever, I'm not trying to focus on that. I'll just let my tennis talk."
The frosty handshake certainly sent tennis twitter into overdrive as journalists and fans picked up on the ice-cold exchange — while Aussie star Nick Kyrgios also took Kerber to task.
The three-time grand slam champion paid tribute to Andreescu on social media but Kyrgios was quick to point out her hypocrisy.
Meanwhile the result left tennis analyst and former men's star Brad Gilbert in awe of what Andreescu is doing right now.
"Kerber baby can defend unbelievable, but right now Bibi Andreescu's 'fearhand' can control the centre of the court and she has the ability to play high heavy spin then totally crack it," he wrote on Twitter.
Next up for Andreescu is Estonian Annett Kontaveit, who conquered Australia's Ajla Tomljanovic in the third round — but we eagerly await the next time Kerber and Andreescu meet.