The 27-year-old was given code violation warning for unsportsmanlike behaviour for swearing towards his player's box and spitting on the court in their direction.
The disgusting incident occurred when his opponent secured a break of serve in the fourth set.
"Go home if you're not going to f***ing support me bro," he shouted towards his player's box, which included girlfriend Costeen Hatzi.
It was a wild match for the angry Aussie and it got completely ridiculous during the second set when he told the chair umpire he could smell marijuana coming from the crowd.
At the change of ends at 4-3, Kyrgios was clearly fuming as he sat down and told the chair umpire.
"It was f***ing marajiuana," he said.
"It was smoke. Obviously I'm not going to complain about food.
"Obviously when athletes are running side to side and they have asthma, it's probably not ideal."
The chair umpire responded by making an announcement to the crowd.
"Ladies and gentlemen as a courtesy to the players please refrain yourselves from smoking around the court. Thank you," he said.
Aussie commentator Todd Woodbridge said: "Nick has asked the umpire to remind the crowd about smoking, saying he thinks he can smell someone smoking marijuana, never without drama are we?"
The tournament rules for spectators state that those attending the US Open should "refrain from smoking, as this is a smoke free environment".
Kyrgios at Wimbledon also complained to the chair umpire about a fan in the crowd he claimed had "700 drinks".
The woman has denied the suggestion she was drunkenly distracting him from serving and is suing the tennis star for defamation.
