"I have thought long and hard about this decision; I know how much time and effort is required to commit to a Commonwealth Games and World Cup campaign," Harrison said.
"I pride myself on giving 100% in everything I do, and am proud to be able to hang up the black dress on my own terms.
"Winning gold at the 2006 and 2010 Commonwealth Games are memories that I will always treasure and are two of the standout moments from my career."
A dual-international, Harrison spent three seasons on the international beach volleyball circuit in between stints on the netball court, and also took time away from sport for the birth of her two children.
Netball New Zealand chief executive Jennie Wyllie says Harrison is one of the toughest competitors to ever play for the Silver Ferns.
"Anna has had a decorated international career over a long period of time. She has been a key member of some of the Silver Ferns most feared defensive combinations and is still one of the world's best defenders," Wyllie said.
Harrison will continue to play in the ANZ Premiership for the Northern Mystics as the race to the finals heats up over the remaining rounds.