"We have long been aware of people driving at excess speeds along Marine Parade," Collins said.
"We often take down the registration numbers of vehicles that we see speeding and report them to local police.
"Yet people continue to speed past — sometimes at up to 100 km/h."
Little penguins are protected and under The Wildlife Act 1953, the maximum penalty for killing protected wildlife is a $100,000 fine and up to a year in jail.
Forest & Bird South Canterbury have contacted Timaru District councillors and have launched a petition online to reduce the speed limit in the area.
Collins said it was important for a change to be made by the Timaru District Council due to the increased number of people in the area over summer.
"This is a safety issue - for the penguins that call Caroline Bay home, but also for the people who come to watch them come ashore," she said.
"In the last week alone, we have seen between 50 and 100 visitors each night, and those numbers will only rise in the coming summer months."