During the hearing at Northampton Crown Court, he wrote on a whiteboard to confirm his name and date of birth.
Prosecutors allege Ratana was shot while De Zoysa was handcuffed inside the Croydon custody suite.
During the alleged incident, De Zoyza sustained a gunshot wound to the neck.
He was remanded in custody until his trial, which is expected to last for three weeks.
‘Big in heart’
Ratana, who was of Māori descent, was a highly accomplished rugby coach and just two months away from being eligible for retirement when he died.
Dame Cressida Dick, the Met Commissioner at the time, described Ratana as a “talented officer” who was “big in heart”.
“Matt spent nearly 30 years as a uniformed police officer, serving the public of London,” she said.
“He will be remembered so fondly in Croydon and missed there, as well as in the Met and the rugby world,” she added.