"I don't agree with Treasury's numbers. They've made some very questionable assumptions," Twyford told reporters today.
"Unfortunately, the bean counters in Treasury are somewhat disconnected from reality. The officials in MBIE, who are much closer to the industry and are actually involved with making Kiwibuild happen, they actually estimate that the impact on investment could be as much as $11 billion for the same period," he said.
Finance Minister Grant Robertson backed Twyford, saying he hadn't heard Twyford make the comments.
Steven Joyce, finance minister in the previous National government tweeted that Twyford's comments were "reasonably high on the arrogance scale".
It is not the first time Twyford has clashed with officials over Kiwibuild.
Last November he said Treasury had provided incorrect advice to the Reserve Bank which led the bank to say only 5000 houses could be built a year because private activity would decrease as the programme ramped up.
To reach the Government's total of 100,000 houses in a decade, 10,000 a year need to be built.
National's housing spokeswoman Judith Collins said Treasury was the third government ministry to tell Twyford he was wrong on Kiwibuild.
"Mr Twyford has now been told by the Reserve Bank that the impact of KiwiBuild on residential construction will be offset by a halving of private sector capacity, by MBIE that he won't deliver the KiwiBuild houses for the $2 billion he claimed and now by Treasury that KiwiBuild is falling apart.
"But he's arrogantly said all those experts are wrong and he's right," Collins said in a statement.