Harrison allegedly shot dead Sio Matalasi during a gang-related confrontation. Mr Matalasi's father has called for the photo to be withdrawn, accusing the gallery of immortalising Harrison.
Mr Langsford said the image of Harrison was taken four years ago - roughly three years before Harrison is accused of murdering Mr Matalasi. The body of work had taken Rotman seven years to compile.
"It is just a bloody unfortunate set of circumstances. In the context of the show it is just another image," Mr Langsford said. "No one would even know who this guy was if you guys [the Herald] hadn't have grabbed hold of it ... We've removed that image from our website and taken it off all publicity. And had we known we would have used another image, of course."
Rotman last year received the prestigious $25,000 Marti Friedlander Photographic Award for his work depicting gang members. The New York-based photographer flew in for the exhibition yesterday.
"He knew there would be some negative response but I don't think he felt it would be as much as we've had," Mr Langsford said. "By the same token, we've had people turn around and say 'good on you, you have to do it whether you like it or not'. We are not here to show pretty pictures. Sometimes art is challenging and does create some controversy."
Last night Rotman said he felt sympathy for Mr Matalasi's family but he would only deal with them directly.
"It is not something I take lightly," he told Seven Sharp. "I really feel for them. It is a terrible occurrence but it is not something I am going to do through the media."
He said the timing of Harrison's court appearances was unfortunate "for a number of reasons but I am not going to censor my work".