"Dr Mortimer fraudulently and knowingly concealed his use of his own genetic material in the procedure," according to the complaint. "Dr Mortimer knew Kelli Rowlette was his biological daughter but did not disclose this."
To increase chances of fertility, the couple agreed to mix the husband's sperm with other matching donors, noting they preferred college students more than 180cm tall.
Ms Rowlette is accusing Dr Mortimer, who later also delivered her, of making false statements to her parents about following their wishes and never revealing he was the biological father.
Ms Rowlette initially dismissed the Ancestry.com test that determined Dr Mortimer was her father, but later found her birth certificate with his signature, the Daily Beast reported.
She is suing the retired doctor claiming medical negligence, battery and fraud.