Dr Chris Hamilton, from the Florida Museum of Natural History, who named the spider, said: "We often hear about how new species are being discovered from remote corners of the Earth, but what is remarkable is that these spiders are in our own backyard.
"With the Earth in the midst of a sixth mass extinction, it is astonishing how little we know about our planet's biodiversity, even for charismatic groups such as tarantulas."
The team spent more than a decade searching for tarantulas in locations ranging from scorching deserts to frigid mountains in the American southwest.
In total the scientists looked at nearly 3000 specimens - the most comprehensive taxonomic tarantula study ever conducted.
Their findings, published in the journal ZooKeys, revealed 29 US tarantula species, 14 of which were new to science.
The spiders ranged in size with some being no bigger than a small coin and others, like A. johnnycashi, having leg spans of 15cm or more.
Their bite, though venomous and painful, is not considered dangerous.
Dr Hamilton stressed that despite their fearsome appearance, US tarantulas were harmless. He described the creatures as "teddy bears with eight legs".
-AAP