Joining Robbie is fellow Australian Jai Courtney (Captain Boomerang), while big names like Will Smith ( Deadshot) Ben Affleck (Batman) and Cara Delevingne (Enchantress) fill out the cast.
Set to Queen's Bohemian Rhapsody the trailer is filled with insights into the warped minds of the villains and the powers they possess.
Earlier fans were treated to a new poster depicting the characters in full costume.
The Joker is shown with a tattoo saying "Damaged" positioned right on his forehead and stands away from the rest of the cast.
The theatrical trailer debuted Wednesday at 4pm (NZ time), with fans desperate for new information.
"The entire world as been waiting for this," fan Enzo Sanchez wrote on Facebook.
Within seconds of the release of Suicide Squad's new trailer, people took to social media doting over the comic book movie.
The film is set to be released in New Zealand on August 4.
-AAP