Scarlett Johansson gets slimed during MTV Awards by her husband Colin Jost

ScarJo was slimed by her husband while accepting her MTV Award. Photo / MTV

Someone is about to be in the doghouse.

Colin Jost pulled the ultimate prank on his wife Scarlett Johansson during her acceptance speech during the MTV Movie and TV Awards.

While the glamorous actress was accepting the Generation Award, her husband came on over and poured slime all down her hair and face.

Expressing shock while her smiling husband slimed her, she finished her speech.

"What the f***?" she yelled.

"MTV, you got slimed," said Jost.

Scarlett accepted the Generation Award. Photo / MTV

"That's Nickelodeon," Johansson blurted.

"I'm very, very sorry," he said, as he promised to "get a towel".

Johansson asked her husband where he got the slime from, to which he answered, "Amazon".

The actress was joined by a slew of other actors and actresses who have received the Generation Award, including Sandra Bullock, Jim Carrey, Reese Witherspoon, Adam Sandler and Tom Cruise.

A slimy surprise. Photo / MTV

"Thank you so much to my fans for riding the way with me and supporting my career so I can continue to have the good fortune to pursue the job that is my passion, and I realise what an absolute gift it is to have the opportunity to do what I love," Johansson said in her speech. "I couldn't do it without your continued support."

Johansson and Jost announced their marriage in October through an Instagram post on the page for Meals on Wheels America.

The post showed a picture of a Staten Island Ferry with the words "Jost Married" across the image.

The couple met through Saturday Night Live.