Timberlake made Biel wear the outfit after an old interview resurfaced last week in which Biel confessed she wasn't a fan of NSYNC.
During an appearance on The Tonight Show with Jimmy Fallon last week, Biel squirmed as the talk show host played a clip from a 1999 interview of hers.
"To be honest, I don't really listen," she said in the interview when asked if she was a fan of the boy band. "I mean, I know of them, of course, and I've heard of them and I've heard the music. I don't think I own any of their CDs. I'm not a huge fan. But, I mean, cool, I guess."
When the vintage clip ended, Biel said to Fallon, "I am so screwed now."
It didn't take Timberlake long to get payback. He shared a photo of his wife's Halloween costume on Instagram and wrote: "This is what happens when your wife admits on TV that she doesn't know any NSYNC songs."
Biel and Timberlake were just two of the big name stars that attended the Casamigos Halloween party on the weekend along with Cindy Crawford, Laverne Cox, Nina Dobrev, Ellen Pompeo, Gerard Butler and Paris Hilton.