Matafeo thanked her director Phoebe Bourke for taking a "punt" on her.
"Three years ago my director [Phoebe Bourke] took a punt on taking me up to Edinburgh with [Berk's Nest] without ever having met me. I love her and everyone at Berk's Nest. The hardest workers and the best people - thank you Phoebe, Owen, Katie, Jenny and Maria."
Last year's winner of the Edinburgh Comedy award was Hannah Gadsby, who went on to become a Netflix phenomenon with her acclaimed stand-up special Nanette.
The winner of the top award receives Β£10,000 ($19,276).