Fonterra's business in Chile is the focus of an investigation by the South American country's prosecutor.

The probe follows a complaint from a shareholder of Fonterra subsidiary Prolesur about commercial dealings between Prolesur and another Fonterra Chile subsidiary, Soprole.

Chilean charity foundation Isabel Aninat, a 13.6 per cent shareholder in Prolesur, has made a complaint against Fonterra's appointed directors on the Prolesur board.

Prolesur, based in southern Chile, is a Fonterra ingredients business. Soprole, a consumer product business operating in the north, says it sources about 30 per cent of its daily fresh milk from Prolesur.

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