Berkshire Hathaway, which operates primarily as a holding company with shareholdings across sectors, warned most of its businesses had been negatively affected by the pandemic's downturn.
Despite the downturn, the conglomerate's cash pile swelled to US$137bn. The company has been holding off on new acquisitions since the downturn, unlike during the 2008 financial crisis, when it went on a buying spree.
Analysts warned his unwillingness to invest suggested he believed the market had further to fall.
Berkshire Hathaway's investor conference normally attracts tens of thousands of shareholders to its base in Omaha, Nebraska.
This time, Buffett, 89, addressed investors via video link.
Late on Saturday, Buffett told a virtual meeting of investors: "We made that decision in terms of the airline business. We took money out of the business basically even at a substantial loss."
He added: "The world changed for airlines and we wish them well."