From here, things got even more convoluted.
When Ms Carlson sent the item to NASA in the hope of obtaining further information about the history of her purchase.
The part number printed inside of the bag correlated to the "Contingency Lunar Sample Return Decontamination Bag" listed in the Apollo 11 Stowage list and the dust was confirmed to be from the moon.
Realising the object was in fact something NASA wasn't aware it had lost, the space agency refused to return the object to Ms Carlson and asked the court to rescind the auction because it believed it was the dust's rightful owner.
In June last year, Ms Carlson launched legal action against NASA for wrongful seizure of property in U.S. District Court in Chicago, with support from former special agent at NASA's Office of the Inspector General Joseph Gutheinz.
"It's an incredible piece of history, and losing it was a colossal mistake for NASA," he told the Wall Street Journal.
"The government sold off a national treasure and then got seller's remorse ... but Nancy Lee bought it fair and square."
In December, U.S. District Judge J. Thomas Marten ruled in favour of Ms Carlson and ordered NASA to return the dust.
"Ms. Carlson's standing as a bona fide purchaser gives her priority over NASA's asserted claim to the property," he ruled.
Sotheby's Cassandra Hatton - a representative from the premium auction-house listing the item - said she expects a lot of interest in the item, which is tipped to sell for US$4m.
"This is a once-in-a-lifetime event," she told Chicago Tribune.
"[It might be] the only privately held object of its kind in the world."
Ms Carlson said she plans to donate some of the proceeds to charity.