However, Ma prompted controversy recently after he endorsed the overwork culture, posting online in April that "to be able to work 996 is huge bliss", arguing that milestones only come with great sacrifice. Other Chinese tech executives such as Richard Liu, the chief executive of JD.com, an e-commerce firm, agreed, saying that slackers were not "brothers".
All this inflamed an already fraught discussion in industry about the societal pressure to give up everything for a job and an employer.
In March, a group of developers started a project online called "996.icu", alluding to the idea that working such hours could land someone in the intensive-care unit.
According to Chinese law, businesses are only supposed to let staff work eight hours a day, or 40 hours a week, with overtime capped at 36 hours a month.
Ma's latest remarks appear to roll back his earlier "996" endorsement, while still falling in line with Alibaba's company culture, which has long prided itself on maintaining the feeling of a start-up despite ballooning to an early US$500 billion ($760.6b) in market value.
Each year in May, the company celebrates its employees with a day called "Aliday," where couples who work for Alibaba take part in a group wedding ceremony. The company flies international employees to its Hangzhou headquarters to participate.
Ma's comments caused a stir online, with some criticising the businessman's comments as "disgusting".
Others wondered whether he was attempting to encourage a baby boom given China's looming demographics challenge.
Experts have warned that China's population will start to shrink over the next decade. The country's birth rate hit a record low of 15 million births last year, the lowest number of babies born since 1949.
By 2050, about a third of the population will be elderly, straining state coffers and leaving fewer people of working age.