They have launched an online petition, Save the Cinque Terre from Mass Tourism, calling for the number of visitors to the World Heritage area to be managed better.
One of the hundreds of people who have signed the petition, which was launched a few days ago, wrote: "They were once a paradise but now they have become hell."
The petition organisers wrote that "the Cinque Terre has boomed in popularity among international travellers and is suffering the consequences of unsustainable - and unrestricted - mass tourism".
Visitors arrive not only by bus but also by train - the villages are served by a railway line that winds in and out of tunnels carved through the steep cliffs.
There are few public toilets in the Cinque Terre villages and local inhabitants complain of tourists urinating " or worse " in their back gardens and in doorways.
"We want to stagger the arrival of tour buses so that they don't all arrive at the same time," said Franca Cantrigliani, the Mayor of Riomaggiore, one of the five villages. "The responsibility for that lies with the port authority of La Spezia and the cruise ship companies."
The cruise industry said it was "saddened" to hear that the inhabitants of the Cinque Terre felt overwhelmed by the numbers.
Francesco Galietti, the national director of the Italian chapter of the Cruise Lines International Association, said the cruise sector in Italy had created more than 100,000 jobs and generated revenue of more than 4.5 billion ($7.9 billion) last year.