It didn't take long for Trump to hit back at Ryan.
"Paul Ryan should spend more time on balancing the budget, jobs and illegal immigration and not waste his time on fighting Republican nominee," Trump wrote on Twitter.
Ryan, who had expressed disgust over the tape and cancelled a campaign event with Trump at the weekend, did not completely cut ties to Trump, later clarifying on the conference call he was not withdrawing his endorsement.
Many Republican members of Congress are concerned that Trump's chaotic campaign could ruin their chances of holding their majorities in the House and Senate and could inflict long-term damage to the party.
According to Reuters, nearly half of all 331 incumbent Republican senators, Congress members and governors have condemned Trump's remarks, and roughly one in 10 have called on him to drop out of the race.