The chief prosecutor of the Sicilian port city of Catania, Carmelo Zuccaro, said he also suspected that there may be direct communication between Libya-based smugglers and members of charity-operated rescue vessels.
Non-governmental organisations deny any wrongdoing, saying they are simply looking to save lives, but they are facing criticism in Italy, which has taken in about half a million migrants since the start of 2014.
A parliamentarian with the right-wing Northern League party on Sunday accused the NGOs of acting as a "taxi service", bringing migrants straight to Italy rather than to closer nations, such as Tunisia and Malta.
"If the government does not decide to put a brake on these NGO boats, we will find ourselves overrun by tens of thousands of African immigrants by the end of the year," lawmaker Paolo Grimoldi said.
Migrants who have come this year have told of increasing violence and brutality in Libya, where rival factions battle for power and people smugglers operate with impunity since the 2011 overthrow of former leader Muammar Gaddafi.
- AAP