Footage shows Putin shooting in his suit and tie while sitting at a desk at a Patriot Park firing range in Moscow region.
He showcased his sniper skills as he blasted Britain over the latest claims that he poisoned his enemies in Salisbury.
His spokesman dismissed as absurd claims that Putin had ordered the assassination of a part-time model with rat poison in a Salisbury restaurant.
Anna Shapiro, the alleged victim of the attack, said: "I was targeted by Putin's henchmen.
"They want me dead as I oppose Putin and have turned my back on my country."
Putin's spokesman Dmitry Peskov said: "I don't think there is any sense in commenting on such reports."
He told journalists: "The absurdity of the most part of information coming from Salisbury is evident."
The sniper rifle is made by Kalashnikov Concern which was created with the merger in 2013 in a merger of two Soviet-era plants – Izhmash and Izhevsk Mechanical Plant.