Those who watched the pre-recorded show said Youssef had largely focused on the station's policy, mocking its statement criticizing his first show that satirized nationalist fervor that nearly idolized the military and its leaders.
"The station's decision to suspend the program had reflected a very negative image to the world about the democratic environment and the freedom of expression in Egypt" after the popularly backed coup that ousted Islamist President Mohammed Morsi from office in July, QSoft said in a statement.
Youssef's program has often stirred controversies, making him the target of many legal complaints. Authorities already investigated him for the first episode on charges of disrupting public order and insulting Egypt and military leaders.
Youssef's popularity peaked during Morsi's rule, when he was the target of weekly jokes mocking him and his Islamist allies for mixing religion and politics. Youssef was also briefly detained and released on bail under Morsi on accusations of insulting the president and Islam.
It is not yet clear if Youssef will go to another station.