The detainee is accused of aggravated human trafficking, conspiracy to commit aggravated homicide and aggravated fraud.
Police say that over $145,000 has been claimed in insurance following the deaths of several victims.
It is believed that Flores Acosta has been operating the violent scam for six months. Two other female gang members have also been arrested, one of whom is the Black Widow's sister.
The gang recruited attractive girls in shopping centres and universities with the promise of taking them to the US.
However, after the deaths of their new husbands, they were instead taken to Mexico where they were forced into prostitution.
Flores Acosta said of the accusations: "I declare myself innocent. I have never worked at home. I sell vehicles and I am a stylist."