Concealed weapons permit holder Russell let off three shots all of which hit the attacker.
Strother was later pronounced dead after being rushed to hospital.
The state attorney's office confirmed that the killing was justified as the life of the officer was in danger.
Lee County Sheriff's Office praised Russell's action hailing him as a 'hero'.
A note from Sheriff Mike Scott:
Earlier this week, two heroes met on I-75 and while they had never met before, one...
Posted by Lee County Sheriff's Office on Wednesday, 16 November 2016
In a Facebook post, officer Mike Scott said: "Earlier this week, two heroes met on I-75 and while they had never met before, one would save the others life.
"Ironically, this was the first day in a while that Deputy Bardes was alone on patrol because as a Field Training Officer, he typically has a recruit riding with him to learn the business."
He heaped further praise on Russell, adding: "I thank the hero that recognised the imminent threat, rushed to Deputy Bardes' aid, and ultimately stopped that threat.
"In a day and age where race is a near instant focus for media and other pundits in police incidents, the fact is that this hero happens to be a man of colour who stopped another man of colour from further harming or killing a white cop..."
"...thereby reminding us that black lives matter, blue lives matter, and indeed all life matters."
While Russell's gun was taken as evidence it was immediately replaced by gun store Shooting Straight, Scott confirmed.
Not a single 911 call was reportedly made during the astonishing incident.