Floyd worked as a security guard inside the club last year.
Santamaria is unsure whether they ever met, but they did work in close proximity every Tuesday night.
She said Floyd was well liked by club patrons and beloved in the Latin community, as he worked at one other Latin club.
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Chauvin has been fired from the police department following his charges in relation to Floyd's death.
She said customers never complained about Chauvin but did have comments about how he handled them.
Her club burned down last week during the protests over Floyd's death.
According to CBS, Santamaria has reached out to Floyd's family.
The family's lawyer, Benjamin Crump, told CBS' "Face the Nation" that the connection between the two means Chauvin should face tougher charges.
"That is going to be an interesting aspect to this case and hopefully upgrading these charges to first-degree murder because we believe he knew who George Floyd was," the lawyer said. "We think that he had intent."
Santamaria says she would like to see Chauvin punished.
"We have to make Derek Chauvin an example so that police around the country realise that it's not okay, and they're not going to get away with it and there will be repercussions. Otherwise it's going to continue to happen," she said.