"I'm worried that there seems to be a bit of a free-for-all at the moment where it seems okay to start attacking the Prime Minister based on her gender."
Gillard has expressed concern Sattler's questioning sent the wrong message to young girls, who should be encouraged to enter public life.
Sattler on Thursday had asked Gillard to test some "myths, rumours, snide jokes and innuendos", following up with, "Tim's gay. That's not me saying it, that's a myth".
"Well, that's absurd," Gillard responded.
"Yeah, but you hear it, he must be gay, he's a hairdresser ... you can confirm that he's not?" Sattler asked.
"Oh, Howard, don't be ridiculous, of course not," the Prime Minister replied.
Also this week it was revealed Ms Gillard's body had been mocked in a menu produced by a Brisbane restaurateur for a Liberal-National Party fundraiser.
- AAP