For Four Paws Director of Project Development Dr Amir Khalil getting the animals to the Turkish border was a great relief.
"I'm very happy that the animals are here, but we still have another 1200km to go until we can say we have done our job," he said.
It's the second time Four Paws has successfully freed zoo animals from a warzone.
Three months ago the group launched another high-risk operation across the border in Mosul in Iraq to rescue a lion and bear.
New Zealand cameraman Anton Leach was part of the group that helped get the animals to safety and said any such operation was fraught with danger.
"We are a target. We are people who Isis would like to capture and extort money out of. It's pretty scary," he said at the time.
Four Paws says they're thrilled about their latest success but say more animals are still trapped at the partially destroyed zoo in Syria and will also need to be rescued.