Alhambra Nievas became the first female referee to take charge of a men's international in Europe.
Last month it was announced that Neville, who has only been refereeing for three years would join Nievas and Cox as a full-time professional referee, leaving her day job as a rugby development officer at the Limerick Institute of Technology.
Neville has already been appointed as an assistant referee at Champions Cup, Challenge Cup, Continental Shield levels and has regularly been appointed as assistant referee at Guinness Pro14 fixtures. She also took charge of the Women's Rugby World Cup final between New Zealand and England in August.
Due to her former Test rugby career, Neville is regarded as having an excellent sense of empathy with players when officiating.
In advance of her Challenge Cup debut, she will officiate as an assistant referee at the Barbarians versus Tonga game at Thomond Park in her home city of Limerick this coming Friday, and at the Spain versus Canada and the France versus Japan internationals later this month.
Neville made more history last weekend when she became the first woman to referee a Principality Premiership game in Wales. The Welsh Rugby Union appointed her to officiate Pontypridd's 45-12 victory over Ebbw Vale.