31-year-old Rozenstruik refused to touch gloves before the fight, leaving his opposer with outstretched arms.
The bout proceeded at a slow pace, with Overeem slowly compiling points — it was revealed after the fight he had won the first four rounds on all three official scorecards.
Overeem fatigued in the final round, and only needing the survive until the final buzzer, was eventually rattled by an explosive Rozenstruik in the dying seconds.
After managing to get back onto his feet, by referee Dan Miragliotta rushed in to end the fight with four seconds remaining.
Rozenstruik's impressive record now stands at 10-0, with four wins in the Octagon, all via knockout.
