"[There are] no issues, they've just had enough [practice]. We won't be going out over the weekend either. Nothing before Monday," the spokesperson said.
Races around a practice course were set for today but Luna Rossa - as well as INEOS Team UK and American Magic - decided not to participate.
The latest withdrawal will further fuel speculation that the British challenger is experiencing serious issues with their boat with the team having very little racing over the past week.
American Magic, also a non-starter on Thursday, had impressed on Tuesday, with helmsman Dean Barker displaying some adept handling and confidence in tight situations, but some unspecified issues with their boat Patriot meant they couldn't take part.
Teams are expected to take part in practice races on Monday and Tuesday next week, before the start of the World Series on Thursday.
Enjoy smooth sailing to the Cup with Auckland Transport
• Avoid traffic congestion and parking niggles and download the AT Mobile app to plot your bus, train or ferry ride to race venues before you leave home.
• Make sure your AT HOP card is in your pocket. It's the best way to ride to the Cup
• For more ways to enjoy race day, visit at.govt.nz/americascup