A regular starter in recent years, Osborne makes way for Tevita Li from the Blues in a possible pointer towards Brown's thinking.
Hookers Ash Dixon (back) and Greg Pleasants-Tate (knee) weren't considered because of injury, along with five-eighth Marty Banks (knee).
The Highlanders lost their opening trial match 50-33 to the Crusaders two weeks ago.
They reached the quarter-finals at last week's Brisbane Global Tens, eliminated by Hurricanes.
Highlanders: Ben Smith, Waisake Naholo, Malakai Fekitoa, Rob Thompson, Tevita Li, Lima Sopoaga, Aaron Smith, Luke Whitelock, James Lentjes, Liam Squire, Jackson Hemopo, Tom Franklin, Siate Tokolahi, Liam Coltman, Daniel Lienert- Brown.
Reserves: Aki Seiuli, Craig Millar, Siosuia Halanukonuka, Guy Millar, Adrian Smith, Josh Dickson, Alex Ainley, Elliot Dixon, Gareth Evans, Dillon Hunt, Kayne Hammington, Josh Renton, Fletcher Smith, Sio Tomkinson, Teihorangi Walden, Matt Faddes, Pat Osborne, Jason Emery.
-AAP