Court documents lodged in the US claim Hayne took the woman, referred to as Ms V, home in an Uber and she passed out in the car "due to her extreme level of intoxication".
The victim says she was a virgin at the time and wasn't able to give consent to sexual intercourse due to her level of intoxication.
Hayne says he previously provided all reasonable assistance to US authorities when the woman went to police in May 2016.
In a statement from his Sydney lawyer he "unequivocally and vehemently" denied the allegations.
Hayne admitted the pair "hooked up" after being contacted by one of her friends on Instagram.
It's understood the NRL star would be expected to return to the US if the woman is granted a jury trial as she's demanding.
The woman, who claims she didn't immediately report the incident for fear of being discredited or blamed, is seeking monetary compensation for "emotional pain, anguish and severe distress".
Both the Eels and 49ers denied prior knowledge of the matter and the NRL's integrity unit is monitoring the case. Hayne was expected to face the NRL judiciary, who are understood to be keen to know why he failed to alert the governing body that he'd been involved in a criminal matter in the US before he signed with the Gold Coast Titans last year.