The Telegraph reported: "The sound of the bottle could heard of video footage just after the starter told the runners to "set"."
After the race, American bronze medallist Justin Gatlin told reporters: "It was a little distraction and I didn't know what it was.
"But when you're in those blocks and the whole stadium's quiet you can hear a pin drop.
"You just have to block it out and go out there and do what you got to do. You can't complain about that, the race went on and it was a great race."
Bolt, who won the race in a new Olympic record, said he knew nothing of the incident.
"I don't know who would have done that."
Yohan Blake who took the silver said he, too, was so focused he heard nothing,
"I was so focused on just running to the line."
Police said in a statement that no-one was injured in the bottle throwing incident.
A man has been arrested on a charge of causing a public nuisance. The charge relates to shouting abuse at competitors and throwing the bottle.
Organisers of the London Games are also investigating.