A first violation will require the club's employees or medical team members involved to attend remedial education along with a possible maximum fine of $US150,000 ($NZ214,000) against the club.
Subsequent violations will result in a minimum $US100,000 ($NZ142,000) fine.
If the parties agree that a violation involved aggravating circumstances, the club shall be subject, in the first instance, to a fine no less than $US50,000 ($NZ71,000).
NFL Commissioner Roger Goodell will determine discipline for subsequent violations involving aggravating circumstances.
And if Goodell determines that the club's medical team failed to follow the protocol due to competitive considerations, he may require the club to forfeit draft picks and impose additional fines.
The NFL has been facing criticism over player safety and the link between football and concussions.
The concussion protocol came under renewed scrutiny last season when Los Angeles Rams quarterback Case Keenum was allowed to continue playing after suffering a concussion in a game.
Last November, Keenum sustained a head injury on the field and struggled to get up off the turf after his head hit the ground but he remained in the game and later gave up a fumble that cost his team that game.
The NFL said at the time it would review why Keenum was not taken off the field for evaluation by a team doctor or an independent neuro-trauma physician as required by its concussion protocols.
- AAP