Among those sitting the trip out are experienced midfielder Kayla Whitelock and first choice goalkeeper Sally Rutherford.
"Darwin gives us the chances to continue looking at players and how they perform during high intensity games," Black Sticks coach Mark Hager said.
"We see the series as a valuable and critical part of our build up to the Champions Trophy and also Rio, which is what all are preparations are building towards this year."
The Black Sticks squad: Georgia Barnett, Emily Gaddum (Central), Sam Charlton, Rose Keddell (Midlands), Sophie cocks, Pippa Hayward, Rachel McCann, Olivia Merry (Canterbury), Liz Thompson, Julia King, Amelia Gibson, Charlotte Harrison (Auckland), Petrea Webster, Kirsten Pearce (North Harbour), Ella Gunson, Stacey Michelsen, Brooke Neal (Northland), Anita Punt (Capital).